Although the level of glucose is the contributing factor and the diagnostic marker for type 2 diabetes, there is an imperative need to look "beyond glucose" and look for additional biomarkers that could better predict the risk of diabetes and their diagnosis with a precisionexact
Human serum albumin is one of the most abundant proteins of plasma that is easily glica.Therefore, it has been suggested to glucosylated albumin as an additional marker to control the glycemic state in people with type 2 diabetes.
Scientists from the National Laboratory of Chemistry (Pune, India) collected blood samples from a specialized diabetes clinic, as well as in healthy controls.Diagnostic parameters were measured, including fasting blood glucose, HBA1C, glucose tolerance test, postprandial blood glucose, lipids, urea, creatinine and microalbuminuria.The equal volumes of two plasma samples with similar HBA1C (deviation of less than 0.2%) were grouped, and three of these samples of plasma meetings in each group were used for triplicate analysis, using the technique of spectrometry techniquemasses.
The equipment used the mass spectrometry, accurate, high -resolution (HR/AM), which has raided the way for the creation of the ion fragments library, for the quantification of the glycosylated peptides of the albumin in the context of thediabetes.Glucation is a non -enzymatic chemical reaction between glucose and proteins that lead to the formation of advanced glucation products (Ages), which have been involved in the pathogenesis of type 2 diabetes and its complications.All samples were analyzed in the mass spectrometer, q Exactive Hybrid Cuadrupolo-Gorp (Therm Scientific; Waltham, Ma, USA).
MUTHUSWAMY BALASUBRAMANYAM, PHD, main author of the study, said: “While previous studies quantified only the modifications of the modified lysine of Amadori (LMA), our study, also characterized the modified albumin peptides, carboxymetyl-lisin (CML) andCarboxyethyl-lysine (Cel), and this is an important advance since the CML and CEL are the predominant Ages, constituting up to 80% of the total AGEs.
The association of modified peptides of albumin, CML, with prediabetes, diabetes, and microalbuminuria is a clinically relevant and therapeutically important finding. ”The study was originally published online, on August 1, 2015, in Molecular Magazine Cell Proteomics.